Research paper on hptlc
However, users may print, download, or email articles for individual use. From the results of the present investigation it was concluded that the formulation PPC is highly stable and also possess biologically significant phytocomponents which is may be used as an ailment for treating various disease. Rumalla, B. The Rf value of stigmas-terol matched with the Rf value of extract was about 0. HPTLC method was found to be simple, precise, accurate and convenient method for rapid screening of active constituents present in petroleum ether extracts of plant and can be used for analysis and routine quality control of herbal material and formulations containing B. The correlation coefficient of bacoside A was found to be 0. Schwack J Chromatogr A 42 , Guidance on analytical method validation. Kaale, P. From this, solution was applied using Linomat applicator on TLC aluminum plates precoated with silica gel 60 F 10 x 10 cm, 0. Different techniques has been followed to analyze the purity of the raw materials it includes microscopic, macroscopic, Physical, chemical and biological method of analysis. Limit of detection and limit of quantification For the evaluation of limit of detection and limit of quantification different concentrations of the standard solutions of stig-masterol were applied along with chloroform as blank and determined on the basis of signal to noise ratio. Quantitative estimation of lupeol and stigmasterol in costus igneus by high-performance thin-layer chromatography. Akkad, W.
The methanol layers were collected, filtered and evaporated off. All rights reserved. Ojewole, J.
Hence a densitometric HPTLC method has been developed in the present work for quantitation of stigmasterol from petroleum ether extract of dried leaf and stem of B. This plate was dried in a current of air.
Eyob, S. The Journal of Pharmacy and Pharmacology 51, Agnivesha, Charaka, Dridhabala. Pharmaceutical Press, London, A herbarium sample of this plant is preserved and deposited with A.
Preliminary studies on the antiinflammatory and analgesic activities of Bryophyllum pinnatum.
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