Conclusion for amylase lab with starch temperature

Give each student a slice of bread that has been cut in two.

Amylase starch iodine experiment

Background Information: Enzyme are protein molecules, they are biological catalysts. The results of the pH experiment did not clearly show what the best level for the enzyme was. Figure 2. In particular, a higher temperature generally makes for higher rates of reaction, enzyme-catalyzed or otherwise. This makes the foundation of the experiment vulnerable as it tempered with the initial approach that both substances should be of equal temperature. This means that some results were more reliable than others, as groups that only conducted one trial had nothing to compare their results to. An additional limitation is that the quantity of the amylase and starch were not measured by an exact proportion but rather by sight. One mL of pH 7 starch solution was pipetted into three test tubes. This can be determined by creating different pH levels and amylase reactions that measure the speed of the reaction. They are regulated form a stated of a low activity to a high activity and vice-versa. Start by taking the spotting tiles and putting in 2 drops of iodine solution per each hole, fill 8 holes of the spotting tile. If the digestion happened at a fast pace, as it did in room temperature, those would have been vital for the end result. Then, 1 mL of the temperature equilibrated starch was added. They increase the rate of reaction.

Method: 1. The iodine must turn orange when the reaction is complete. This could also be explained due to anomalies which occurred during the experiment and hence limited its accuracy.

Effect of temperature on amylase activity lab report pdf

The room had the window opened and I I closed the window and the temperature of realised that a lot after so the it was hard to the water stop decreasing so fast but as it maintain the temperatures and had to was a cold day and the room was cold the constantly pour more hot water for the temperature where still hard to maintain for water baths. They increase the rate of reaction. If the digestion happened at a fast pace, as it did in room temperature, those would have been vital for the end result. We can only assume that the results of the groups that only made one experiment are right as we have nothing to compare it to. The concentration experiment was much more clear. All three tubes had maltose present. Literature Cited Brooker, Robert J. The second tube changed from blue to yellow. Then, 1 mL of the temperature equilibrated starch was added. There where other people using the same On the last two water baths I grabbed kettle for their water so they took it way to another kettle twhich was not being used many times out of the platform and that and heated the water for my water baths. The pH level that turned orange the fastest is the preferred pH of amylase. Iodine dissolved in an aqueous solution with starch will produce an intense blue-black purple colour and viceversa if starch is not present, the solution will not change its colour. Record the time. The iodine test is used to test for the presence of starch.

Students should determine the optimum temperature for the amylase reaction through multiple trials. This was repeated at one minute intervals. Independent Variable: The rate of reaction between the amylase and starch.

The experiment is repeated at each pH level.

how does temperature affect amylase activity

Corn Seeds Give the students three corn seeds -- one dry, another that has been boiled, and one that has been soaked in water.

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(DOC) Amylase and Temperature experiment